type vii collagen antibody Search Results


mouse monoclonal antibodies against collagen type i  (Developmental Studies Hybridoma Bank)
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rabbit polyclonal antibodies against type i  (Boster Bio)
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goat anti type 1 collagen antibody  (SouthernBiotech)
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mouse monoclonal anti type ii collagen 5  (Chondrex Inc)
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anti col1a1 antibody  (SouthernBiotech)
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Inhibiting Hck in vivo attenuates kidney fibrosis by TGF-β1/SMAD3 signaling in a murine UUO model. UUO was performed on male C57BL/6 mice, gavaged with dasatinib or vehicle. Mice were perfused 3 or 7 days later. (A) Scheme of the experimental approach is shown. (B) WBs of phospho-SMAD3, t-SMAD3 from mouse kidney cortex lysates at 7 days post-UUO are shown for kidneys of PBS and dasatinib-gavaged mice. (C) Immunohistochemistry for anti-Hck (phosphorylation Y410) antibody in mouse kidney confirming dasatinib inhibiting HCK’s activation. Original magnification, ×200. Also, (D) Hck transcription level was increased in 3 and 7 days UUO kidneys by RT-PCR (normalized to GAPDH). (D) mRNA-levels of profibrotic markers at 7 days post-UUO by RT-PCR (normalized to GAPDH), from whole cortices of control and UUO kidneys of dasatinib- and PBS-fed animals (n=5). (E) Representative ×40 images from control and UUO kidneys of dasatinib- and PBS-gavaged mice at 7 days post-UUO. Picrosirius red stain (top row), and <t>COL1A1</t> IF (middle row, red: COL1A1; blue: DAPI), and a-SMA IF (lower row, red: a-SMA; blue: DAPI) are depicted here. (F, G, and H) Morphometric quantification of thresholded ×40 images (n=6 animals; ten random hpfs per animal), showing area of (F) picrosirius red staining, (G) COL1A1 IF, and (H) a-SMA IF as a percentage of total hpf area. (B, C, D, F, G, and H) Values are mean±SEM; *P=0.05, **P<0.001, ***P<0.001 between means; one-way ANOVA with Bonferroni multiple comparison test.
Anti Col1a1 Antibody, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against collagen i  (Developmental Studies Hybridoma Bank)
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Inhibiting Hck in vivo attenuates kidney fibrosis by TGF-β1/SMAD3 signaling in a murine UUO model. UUO was performed on male C57BL/6 mice, gavaged with dasatinib or vehicle. Mice were perfused 3 or 7 days later. (A) Scheme of the experimental approach is shown. (B) WBs of phospho-SMAD3, t-SMAD3 from mouse kidney cortex lysates at 7 days post-UUO are shown for kidneys of PBS and dasatinib-gavaged mice. (C) Immunohistochemistry for anti-Hck (phosphorylation Y410) antibody in mouse kidney confirming dasatinib inhibiting HCK’s activation. Original magnification, ×200. Also, (D) Hck transcription level was increased in 3 and 7 days UUO kidneys by RT-PCR (normalized to GAPDH). (D) mRNA-levels of profibrotic markers at 7 days post-UUO by RT-PCR (normalized to GAPDH), from whole cortices of control and UUO kidneys of dasatinib- and PBS-fed animals (n=5). (E) Representative ×40 images from control and UUO kidneys of dasatinib- and PBS-gavaged mice at 7 days post-UUO. Picrosirius red stain (top row), and <t>COL1A1</t> IF (middle row, red: COL1A1; blue: DAPI), and a-SMA IF (lower row, red: a-SMA; blue: DAPI) are depicted here. (F, G, and H) Morphometric quantification of thresholded ×40 images (n=6 animals; ten random hpfs per animal), showing area of (F) picrosirius red staining, (G) COL1A1 IF, and (H) a-SMA IF as a percentage of total hpf area. (B, C, D, F, G, and H) Values are mean±SEM; *P=0.05, **P<0.001, ***P<0.001 between means; one-way ANOVA with Bonferroni multiple comparison test.
Antibodies Against Collagen I, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagen iii  (SouthernBiotech)
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Inhibiting Hck in vivo attenuates kidney fibrosis by TGF-β1/SMAD3 signaling in a murine UUO model. UUO was performed on male C57BL/6 mice, gavaged with dasatinib or vehicle. Mice were perfused 3 or 7 days later. (A) Scheme of the experimental approach is shown. (B) WBs of phospho-SMAD3, t-SMAD3 from mouse kidney cortex lysates at 7 days post-UUO are shown for kidneys of PBS and dasatinib-gavaged mice. (C) Immunohistochemistry for anti-Hck (phosphorylation Y410) antibody in mouse kidney confirming dasatinib inhibiting HCK’s activation. Original magnification, ×200. Also, (D) Hck transcription level was increased in 3 and 7 days UUO kidneys by RT-PCR (normalized to GAPDH). (D) mRNA-levels of profibrotic markers at 7 days post-UUO by RT-PCR (normalized to GAPDH), from whole cortices of control and UUO kidneys of dasatinib- and PBS-fed animals (n=5). (E) Representative ×40 images from control and UUO kidneys of dasatinib- and PBS-gavaged mice at 7 days post-UUO. Picrosirius red stain (top row), and <t>COL1A1</t> IF (middle row, red: COL1A1; blue: DAPI), and a-SMA IF (lower row, red: a-SMA; blue: DAPI) are depicted here. (F, G, and H) Morphometric quantification of thresholded ×40 images (n=6 animals; ten random hpfs per animal), showing area of (F) picrosirius red staining, (G) COL1A1 IF, and (H) a-SMA IF as a percentage of total hpf area. (B, C, D, F, G, and H) Values are mean±SEM; *P=0.05, **P<0.001, ***P<0.001 between means; one-way ANOVA with Bonferroni multiple comparison test.
Collagen Iii, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti human collagen type iii c terminal  (Proteintech)
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Inhibiting Hck in vivo attenuates kidney fibrosis by TGF-β1/SMAD3 signaling in a murine UUO model. UUO was performed on male C57BL/6 mice, gavaged with dasatinib or vehicle. Mice were perfused 3 or 7 days later. (A) Scheme of the experimental approach is shown. (B) WBs of phospho-SMAD3, t-SMAD3 from mouse kidney cortex lysates at 7 days post-UUO are shown for kidneys of PBS and dasatinib-gavaged mice. (C) Immunohistochemistry for anti-Hck (phosphorylation Y410) antibody in mouse kidney confirming dasatinib inhibiting HCK’s activation. Original magnification, ×200. Also, (D) Hck transcription level was increased in 3 and 7 days UUO kidneys by RT-PCR (normalized to GAPDH). (D) mRNA-levels of profibrotic markers at 7 days post-UUO by RT-PCR (normalized to GAPDH), from whole cortices of control and UUO kidneys of dasatinib- and PBS-fed animals (n=5). (E) Representative ×40 images from control and UUO kidneys of dasatinib- and PBS-gavaged mice at 7 days post-UUO. Picrosirius red stain (top row), and <t>COL1A1</t> IF (middle row, red: COL1A1; blue: DAPI), and a-SMA IF (lower row, red: a-SMA; blue: DAPI) are depicted here. (F, G, and H) Morphometric quantification of thresholded ×40 images (n=6 animals; ten random hpfs per animal), showing area of (F) picrosirius red staining, (G) COL1A1 IF, and (H) a-SMA IF as a percentage of total hpf area. (B, C, D, F, G, and H) Values are mean±SEM; *P=0.05, **P<0.001, ***P<0.001 between means; one-way ANOVA with Bonferroni multiple comparison test.
Rabbit Anti Human Collagen Type Iii C Terminal, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Inhibiting Hck in vivo attenuates kidney fibrosis by TGF-β1/SMAD3 signaling in a murine UUO model. UUO was performed on male C57BL/6 mice, gavaged with dasatinib or vehicle. Mice were perfused 3 or 7 days later. (A) Scheme of the experimental approach is shown. (B) WBs of phospho-SMAD3, t-SMAD3 from mouse kidney cortex lysates at 7 days post-UUO are shown for kidneys of PBS and dasatinib-gavaged mice. (C) Immunohistochemistry for anti-Hck (phosphorylation Y410) antibody in mouse kidney confirming dasatinib inhibiting HCK’s activation. Original magnification, ×200. Also, (D) Hck transcription level was increased in 3 and 7 days UUO kidneys by RT-PCR (normalized to GAPDH). (D) mRNA-levels of profibrotic markers at 7 days post-UUO by RT-PCR (normalized to GAPDH), from whole cortices of control and UUO kidneys of dasatinib- and PBS-fed animals (n=5). (E) Representative ×40 images from control and UUO kidneys of dasatinib- and PBS-gavaged mice at 7 days post-UUO. Picrosirius red stain (top row), and COL1A1 IF (middle row, red: COL1A1; blue: DAPI), and a-SMA IF (lower row, red: a-SMA; blue: DAPI) are depicted here. (F, G, and H) Morphometric quantification of thresholded ×40 images (n=6 animals; ten random hpfs per animal), showing area of (F) picrosirius red staining, (G) COL1A1 IF, and (H) a-SMA IF as a percentage of total hpf area. (B, C, D, F, G, and H) Values are mean±SEM; *P=0.05, **P<0.001, ***P<0.001 between means; one-way ANOVA with Bonferroni multiple comparison test.

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Genomic Analysis of Kidney Allograft Injury Identifies Hematopoietic Cell Kinase as a Key Driver of Renal Fibrosis

doi: 10.1681/ASN.2016020238

Figure Lengend Snippet: Inhibiting Hck in vivo attenuates kidney fibrosis by TGF-β1/SMAD3 signaling in a murine UUO model. UUO was performed on male C57BL/6 mice, gavaged with dasatinib or vehicle. Mice were perfused 3 or 7 days later. (A) Scheme of the experimental approach is shown. (B) WBs of phospho-SMAD3, t-SMAD3 from mouse kidney cortex lysates at 7 days post-UUO are shown for kidneys of PBS and dasatinib-gavaged mice. (C) Immunohistochemistry for anti-Hck (phosphorylation Y410) antibody in mouse kidney confirming dasatinib inhibiting HCK’s activation. Original magnification, ×200. Also, (D) Hck transcription level was increased in 3 and 7 days UUO kidneys by RT-PCR (normalized to GAPDH). (D) mRNA-levels of profibrotic markers at 7 days post-UUO by RT-PCR (normalized to GAPDH), from whole cortices of control and UUO kidneys of dasatinib- and PBS-fed animals (n=5). (E) Representative ×40 images from control and UUO kidneys of dasatinib- and PBS-gavaged mice at 7 days post-UUO. Picrosirius red stain (top row), and COL1A1 IF (middle row, red: COL1A1; blue: DAPI), and a-SMA IF (lower row, red: a-SMA; blue: DAPI) are depicted here. (F, G, and H) Morphometric quantification of thresholded ×40 images (n=6 animals; ten random hpfs per animal), showing area of (F) picrosirius red staining, (G) COL1A1 IF, and (H) a-SMA IF as a percentage of total hpf area. (B, C, D, F, G, and H) Values are mean±SEM; *P=0.05, **P<0.001, ***P<0.001 between means; one-way ANOVA with Bonferroni multiple comparison test.

Article Snippet: For IF, snap-frozen kidney sections were formalin-fixed and treated with anti-COL1A1 antibody (1:100 dilution; catalog no. 1310–01; Southern Biotech) overnight, and subjected to fluorescence microscopy.

Techniques: In Vivo, Immunohistochemistry, Phospho-proteomics, Activation Assay, Reverse Transcription Polymerase Chain Reaction, Control, Staining, Comparison